cell culture human non small cell lung cancer cell lines  (ATCC)

Journal: Cell communication and signaling : CCS

Article Title: Machine learning-aided discovery of T790M-mutant EGFR inhibitor CDDO-Me effectively suppresses non-small cell lung cancer growth.

doi: 10.1186/s12964-024-01954-7

Figure Lengend Snippet: Fig. 3 The cytotoxicity of CDDO-Me on different NSCLC cells. (a) Schematic representation of CDDO-Me identification. (b) H1975, PC9-ER, A549 and HEK 293T cells were treated with CDDO-Me (0.05, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 5, 10, 15 and 20 µM) for 48 h, respectively. Cell viability was examined by CCK-8 assay and shown as relative viability compared to the untreated control. Each test was performed in triplicate. (c) The IC50 values of CDDO-Me in H1975, PC9- ER, A549 and HEK 293T cells were presented as the mean ± SD. (d) Colony formation of H1975, PC9-ER, and A549 cells were measured after CDDO-Me (0, 0.2, 0.4, and 0.6 µM) treatment for 14 days, and photographs of crystal violet-stained colonies were depicted. (e) The statistical result of colony number after CDDO-Me treatment. Data are shown as mean ± S.D., n = 3. One-way ANOVA with Tukey’s multiple-comparisons test, NS no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Cell lines and cell culture Human non-small cell lung cancer cell lines A549 and H1975 were purchased from the American Type Culture Collection (ATCC, CCL-185, CRL-5908), PC9 cell line was purchased from European Collection of Authenticated Cell Cultures (ECACC, 90071810), HEK 293T cell line was purchased from the American Type Culture Collection (ATCC, CRL-3216).

Techniques: CCK-8 Assay, Control, Staining